Functional Epigenetics in the animal model
Dynamics of epigenetic regulation in neuronal development, function and age-associated neurodegeneration
We investigate the dynamics of epigenetic transcriptional remodelling during neuronal development, function and age-associated neurodegeneration of the cerebral cortex. By dissecting interactive networks directing key events driving these processes, the pathophysiology of associated diseases can be better understood. Our work is based on the systemic and functional analysis of transgenic mouse models and diverse cell culture approaches. For the cellular and sub-cellular analysis, we apply a battery of biochemical, molecular and cell biological methods. These include primary neuronal cultures, functional in vitro assays, ex-vivo and in vivo genetic manipulations, methylation studies and life cell imaging. A recently established methodological focus relies on single cell and tissue-based global transcriptome analysis.